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Weber Scientific DB Sterilized Pre-Filled Dilution Bottles

Sterilized Pre-Filled Dilution Bottles:

Weber pre-filled dilution bottles are used to dilute a liquid or particulate sample for the purpose of microbial enumeration on Petri plates.  Our sterile buffers are manufactured per Standard Methods for the Examination of Dairy Products and Standard Methods for the Examination of Water and Wastewater.  This provides an industry standard that is readily accepted by applicable regulatory agencies.

Each bottle is pre-filled to either 99 or 90 mL, buffered to pH 7.2 +0.2, has an overall capacity of 150 mL and is 100% recyclable.  Two formulations are available:

Butterfield’s Buffer is frequently used in the food and dairy industries (APHA, FDA, AOAC or USP methods). Contains purified water, monobasic potassium phosphate and sodium hydroxide for pH adjust­ment.

Phosphate Buffer is the same as Butterfield’s Buffer but also contains magnesium.  This is commonly used in the water and wastewater industries (APHA or EPA methods). Contains magnesium chloride, monobasic potassium phosphate and sodium hydroxide for pH adjustment.

Applications for Butterfield and Phosphate Buffers:
  • Foods & Beverages
  • Water and Wastewater
  • Cosmetics
  • Pharmaceuticals
  • Environmental Samples

Sterilized Pre-Filled 9 mL Dilution Tubes:

We also manufacture sterile pre-filled dilution Tubes available in Butterfield’s Buffer.  These tubes are pre-filled to 9 mL and have an overall capacity of 13 mL.  This provides an advantage in working with smaller volumes in a smaller footprint.

Features & Advantages of Dilution Bottles:



Lot # and exp. date printed on side of bottle

• Solvent resistant ink withstands­­ 70% Isopropyl Alcohol.

PET bottle

• Glass-like clarity which permits meticulous visual inspection   of contents.
• Exceptional strength - virtually eliminates cracking or   breaking in handling or shipping.
• Safer - avoids release of contents if dropped.

Gamma irradiated

• 10-6 sterility assurance level, the highest level available.
• No chance of microbial contamination – none.

Attached cap

• No cap to set down or hold.
• Enhances aseptic handling and reduces chance of    contamination.
• Stays open at a positive 90° angle.
• Leak-proof - samples can be vigorously shaken without fear    of leakage.

Sterility strip attached to cap

• Tamper evident.
• Cap stays securely sealed.
• Can be shipped by ground or air - no transportation limits -    holds up to rough handling.

Color-coded per formulation and volume

• Rapid and accurate identification.
• Recommended when using multiple formulations or fills.
• Advances good laboratory practice.

32 mm neck diameter

• The wide mouth makes it convenient for liquid or solid    samples.

2-year shelf life from date of manufacture

• Long shelf life.

Certificate of Analysis (COA) is available online

This product is made in strict compliance with specifications as published in the most recent edition of APHA Standard Methods for the Examination of Dairy Products; APHA Standard Methods for the Examination of Water and Wastewater; APHA Compendium of Methods for the Microbiological Examination of Foods: FDA Bacteriological Analytical Manual; FDA Milk Laboratory Evaluation Form 2400; United States Pharmacopoeia. Each lot is thoroughly tested in house with toxicity done by an independent testing laboratory and is released only after passing all specifications for pH, sterility, toxicity, fill volume, and package integrity and markings. The COA indicates that each lot passes all of these specifications. Specific results are given for pH and toxicity analysis.

You may obtain a copy of the Certificate of Analysis (COA), by using the Documents tab on this page.

Huge freight savings and faster delivery throughout the United States

Dilution bottles ship out of our Nevada warehouse for delivery to Western states and out of our Missouri warehouse for selected Southern and Midwestern states. Customers in these areas will realize significant freight savings plus shipments will be received sooner.

Eastern states will continue to receive fast and economical service from our facility in New Jersey.

Would you like to evaluate a free sample?

A free case of Weber Dilution Bottles or Dilution Tubes are available!  Please let us know which product you would like to evaluate when making your request.

Products identified by this icon indicate only the first case — of multiple case orders — will be upgraded to UPS 3 Day Select service (where applicable). The remaining cases will ship by ground and you should receive them within 1 - 3 days of receiving your first case.

Item Color Code Fill Formulation Unit of Measure Price Purchase
Red 99 mL Phosphate Buffer Case of 72
Buy 1 $50.62
Buy 12 $43.56
Buy 25 $41.71
336 In Stock 336 In Stock
Blue 90 mL Phosphate Buffer Case of 72
Buy 1 $50.62
Buy 12 $43.56
Buy 25 $41.71
177 In Stock 177 In Stock
None 9 mL Butterfield's Buffer Case of 100
156 In Stock 156 In Stock
Green 90 mL Butterfield's Buffer Case of 72
Buy 1 $50.62
Buy 12 $43.56
Buy 25 $41.71
227 In Stock 227 In Stock
Black 99 mL Butterfield's Buffer Case of 72
Buy 1 $50.62
Buy 12 $43.56
Buy 25 $41.71
1259 In Stock 1259 In Stock

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