Corning® BioCoat™ Matrigel® Matrix Cellware is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in ECM proteins. Its major component is laminin, followed by collagen IV, heparan sulfate proteoglycans, entactin, and nidogen.
Corning BioCoat Matrigel Matrix Cellware is effective for the attachment and differentiation of both normal and transformed anchorage dependent epithelial and other cell types including neurons and oligodendrocytes.
Elicitation of tissue-specific cellular morphology and protein production in epithelial cells
Differentiation of endothelial, muscle, and neuronal cells
Development of three-dimensional matrix model systems
Corning BioCoat Matrigel Matrix Cellware has been used to culture:
Primary human hepatocytes
Mouse pituitary gland tissue
Human urothelial cells
Osteopontin (OPN) deficient rat vascular smooth muscle cells
Formulation: Dulbecco's Modified Eagles' Medium with 50 μg/ml gentamycin. Corning Matrigel Matrix is compatible with all culture media.
Corning BioCoat Matrigel Matric Cellware is quality-control tested for ability to promote neurite outgrowth from chick dorsal root ganglia in the absence of NGF, and is also tested and found negative for bacteria and fungi.
Cellware stable for at least three months at -20°C. Keep frozen until use. Thin layer cellware stable for at least three months from date of shipment when stored at 2° to 8°C.
Compatible with all culture media
Strict quality control ensures consistent results
Effective for the attachment and differentiation of both normal and transformed anchorage dependent epithelial cell types