Experiment includes complete instructions, background information and activity booklet, reagents for up to 30 spoolings, concentrated chromosomal DNA, Methylene Blue PlusTM, concentrated buffer, 5 glass rods. You need: beakers and test tubes, 10 mL pipet with pump or bulb, 95-100% isopropanol (70% isopropanol rubbing alcohol can be substituted), distilled or deionized water, safety goggles and latex or vinyl gloves.