This lab activity involves analysis of an enzyme reaction using a specific enzyme substrate and inhibitor. Students study how the rate of enzyme reaction is dependent on substrate concentration and the influence of agents (inhibitors) and conditions that affect the enzyme reaction such as pH and temperature. Includes components needed for hands-on experimentation for 24-30 students, Student's and Teacher's Guides.

Requires microplate reader; or spectrophotometer and cuvettes; and stopwatch or timer.